Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases

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Mixed alkyl aryl phosphonate esters as quenched fluorescent activity-based probes for serine proteases.

Activity-based probes (ABPs) are powerful tools for the analysis of active enzyme species in whole proteomes, cells or animals. Quenched fluorescent ABPs (qABPs) can be applied for real time imaging, allowing the visualization of dynamic enzyme activation by fluorescent microscopy. Unfortunately, qABPs are only available for a few enzymes. We here describe the design and synthesis of qABPs for ...

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ژورنال

عنوان ژورنال: Organic & Biomolecular Chemistry

سال: 2015

ISSN: 1477-0520,1477-0539

DOI: 10.1039/c4ob02444c